{"id":117116,"date":"2022-05-23T11:04:56","date_gmt":"2022-05-23T15:04:56","guid":{"rendered":"http:\/\/stateofthenation.co\/?p=117116"},"modified":"2022-05-23T11:07:54","modified_gmt":"2022-05-23T15:07:54","slug":"117116","status":"publish","type":"post","link":"http:\/\/stateofthenation.co\/?p=117116","title":{"rendered":"

OPERATION MONKEYPOX:<\/b> Was China just set up again by the Zio-Anglo-American bioterrorist arm of NATO (aka North Atlantic Terrorist Organization)?!<\/i><\/h2>"},"content":{"rendered":"
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Photo from the Daily Beast.<\/p><\/div>\n

The Infamous Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating \u2018Contagious Pathogens\u2019.<\/h1>\n
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Are we here because of China\u2019s experiments again?<\/h3>\n

by Natalie Winters
\nThe National Pulse<\/p>\n

The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a \u201ccontagious pathogen,\u201d The National Pulse can reveal.<\/p>\n

The study was first\u00a0published<\/a> in February 2022, just months before the latest international\u00a0outbreak<\/a> of monkeypox cases which appear to have now reached the United States.<\/p>\n

The paper, which was authored by nine Wuhan Institute of Virology researchers and published in the lab\u2019s quarterly scientific journal<\/a>\u00a0Virologica Sinica,\u00a0<\/em>also follows the wide-scale use of Polymerase Chain Reaction (PCR) tests to identify COVID-19-positive individuals.<\/p>\n

Researchers appeared to identify a portion of the monkeypox virus genome, enabling PCR tests to identify the virus, in the paper: \u201cEfficient Assembly of a Large Fragment of Monkeypox Virus Genome as a qPCR Template Using Dual-Selection Based Transformation-Associated Recombination<\/em>.\u201d<\/p>\n

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Monkey pox viruses \u2013 referred to as \u201cMPXVs\u201d in the paper \u2013 have strains that are \u201cmore pathogenic and [have] been reported to infect humans in various parts of the world.\u201d<\/p>\n

\u201cQuantitative polymerase chain reaction (qPCR) is the gold standard for the detection of orthopoxvirus (including MPXV). For pan-orthopoxviruses detection, the E9L (DNA polymerase) gene has been shown to be an excellent target for qPCR assays. For MPXV detection, Li et al. reported that the C3L (complement-binding protein) gene could be used as the qPCR target for the MPXV Congo Basin strain,\u201d explained the paper before noting that China lacked sufficient genetic information on the virus for PCR detection:<\/p>\n

\u201cSince MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable. In this report, we employed dual-selective TAR to assemble a 55-kb MPXV genomic fragment that encompasses E9L and C3L, two valuable qPCR targets for detecting MPXV or other orthopoxviruses.\u201d<\/p><\/blockquote>\n

\u201cThe primary purpose of assembling a fragment of the MPXV genome is to provide a nucleotide template for MPXV detection,\u201d reiterated the study, which relied on the process of transformation-associated recombination (TAR) to isolate a genomic fragment of the monkeypox virus.<\/p>\n

\u201cAs an efficient tool for assembling large DNA fragments up to 592 kb in length, TAR assembly has become essential for preparing infectious clones of large DNA\/RNA viruses,\u201d explained researchers.<\/p>\n

The paper acknowledged that TAR \u201capplied in virological research could also raise potential security concerns, especially when the assembled product contains a full set of genetic material that can be recovered into a contagious pathogen.\u201d<\/p>\n

\u201cIn this study, although a full-length viral genome would be the ideal reference template for detecting MPXV by qPCR, we only sought to assemble a 55-kb viral fragment, less than one-third of the MPXV genome. This assembly product is fail-safe by virtually eliminating any risk of recovering into an infectious virus while providing multiple qPCR targets for detecting MPXV or other Orthopoxviruses,\u201d posited researchers.<\/p>\n

The unearthed study follows the Wuhan Institue of Virology conducting<\/a> similar research into strains of bat coronaviruses that could infect humans while admitting its facilities lacked<\/a> proper laboratory safety protocols.<\/p>\n

Read:<\/h4>\n