{"id":75589,"date":"2021-07-25T10:56:06","date_gmt":"2021-07-25T14:56:06","guid":{"rendered":"http:\/\/stateofthenation.co\/?p=75589"},"modified":"2021-07-25T10:58:02","modified_gmt":"2021-07-25T14:58:02","slug":"the-multitargeted-drug-ivermectin-a-scientific-research-paper","status":"publish","type":"post","link":"http:\/\/stateofthenation.co\/?p=75589","title":{"rendered":"The Multitargeted Drug Ivermectin (A Scientific Research Paper)"},"content":{"rendered":"<div class=\"pmc-page-banner whole_rhythm\">\n<div>\n<p><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/corehtml\/pmc\/pmcgifs\/logo-ajcr.gif\" alt=\"Logo of ajcr\" usemap=\"#logo-imagemap\" \/><\/p>\n<map id=\"logo-imagemap\" name=\"logo-imagemap\">\n<area title=\"Link to Publisher's site\" alt=\"Link to Publisher's site\" coords=\"0,0,499,74\" shape=\"default\" href=\"http:\/\/www.ajcr.us\/\" target=\"_blank\" rel=\"noopener noreferrer\" \/><\/map>\n<\/div>\n<\/div>\n<p><!--more--><\/p>\n<div class=\"\">\n<div id=\"mc\" class=\" article lit-style content pmc-wm slang-all page-box\">\n<div id=\"ui-ncbiinpagenav-1\" class=\"jig-ncbiinpagenav\" data-jigconfig=\"smoothScroll: false, allHeadingLevels: ['h2'], headingExclude: ':hidden,.nomenu'\">\n<div class=\"fm-sec half_rhythm no_top_margin\">\n<div class=\"fm-flexbox\">\n<div class=\"fm-citation\">\n<div class=\"citation-default\">\n<div class=\"part1\"><span role=\"menubar\"><a role=\"menuitem\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Am J Cancer Res.<\/a><\/span>\u00a02018; 8(2): 317\u2013331.<\/div>\n<div class=\"part2\"><span class=\"fm-vol-iss-date\">Published online 2018 Feb 1.<\/span><\/div>\n<\/div>\n<\/div>\n<div class=\"fm-ids\">\n<div class=\"fm-citation-pmcid\"><span class=\"fm-citation-ids-label\">PMCID:\u00a0<\/span>PMC5835698<\/div>\n<div class=\"fm-citation-pmid\">PMID:\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29511601\">29511601<\/a><\/div>\n<\/div>\n<\/div>\n<h1 class=\"content-title\">The multitargeted drug ivermectin: from an antiparasitic agent to a repositioned cancer drug<\/h1>\n<div class=\"half_rhythm\">\n<div class=\"contrib-group fm-author\"><a class=\"affpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Juarez%20M%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=29511601\">Mandy Juarez<\/a>,<sup>1<\/sup>\u00a0<a class=\"affpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Schcolnik-Cabrera%20A%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=29511601\">Alejandro Schcolnik-Cabrera<\/a>,<sup>1<\/sup>\u00a0and\u00a0<a class=\"affpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Due%26%23x000f1%3Bas-Gonzalez%20A%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=29511601\">Alfonso Due\u00f1as-Gonzalez<\/a><sup>2<\/sup><\/div>\n<\/div>\n<div class=\"fm-panel half_rhythm\">\n<div class=\"togglers fm-copyright-license\"><a class=\"pmctoggle\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\">Author information<\/a>\u00a0<a class=\"pmctoggle\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\">Article notes<\/a>\u00a0<a class=\"pmctoggle\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\">Copyright and License information<\/a>\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/about\/disclaimer\/\">Disclaimer<\/a><\/div>\n<\/div>\n<div id=\"pmclinksbox\" class=\"links-box whole_rhythm\">\n<div class=\"fm-panel\">\n<div>This article has been\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/citedby\/\">cited by<\/a>\u00a0other articles in PMC.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"sec\"><\/div>\n<div id=\"idm140616623426656\" class=\"tsec sec\" lang=\"en\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><span role=\"menubar\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"menuitem\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/span><\/div>\n<h2 id=\"idm140616623426656title\" class=\"head no_bottom_margin ui-helper-clearfix\">Abstract<\/h2>\n<div>\n<p id=\"__p1\" class=\"p p-first-last\">Drug repositioning is a highly studied alternative strategy to discover and develop anticancer drugs. This drug development approach identifies new indications for existing compounds. Ivermectin belongs to the group of avermectins (AVM), a series of 16-membered macrocyclic lactone compounds discovered in 1967, and FDA-approved for human use in 1987. It has been used by millions of people around the world exhibiting a wide margin of clinical safety. In this review, we summarize the\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0evidences demonstrating that ivermectin exerts antitumor effects in different types of cancer. Ivermectin interacts with several targets including the multidrug resistance protein (MDR), the Akt\/mTOR and WNT-TCF pathways, the purinergic receptors, PAK-1 protein, certain cancer-related epigenetic deregulators such as SIN3A and SIN3B, RNA helicase, chloride channel receptors and preferentially target cancer stem-cell like population. Importantly, the\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0antitumor activities of ivermectin are achieved at concentrations that can be clinically reachable based on the human pharmacokinetic studies done in healthy and parasited patients. Thus, existing information on ivermectin could allow its rapid move into clinical trials for cancer patients.<\/p>\n<\/div>\n<div class=\"sec\"><strong class=\"kwd-title\">Keywords:\u00a0<\/strong><span class=\"kwd-text\">Ivermectin, cancer, drug repurposing<\/span><\/div>\n<\/div>\n<div id=\"__sec1\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec1title\" class=\"head no_bottom_margin ui-helper-clearfix\">Introduction<\/h2>\n<p id=\"__p2\" class=\"p p-first\">The antiparasitic drug ivermectin was initially approved in humans in 1987 to orally treat onchocerciasis, also known as river blindness, caused by the blackfly-transmitted parasite\u00a0<em>Onchocerca volvulus<\/em>\u00a0in poor populations around the tropics, mostly in West and Central Africa [<a id=\"__tag_670054659\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>,<a id=\"__tag_670054554\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b2\" aria-expanded=\"false\" aria-haspopup=\"true\">2<\/a>]. In humans, its use has improved the nutrition, general health and well-being of billions of people worldwide since it was first used to treat onchocerciasis. Not only that, but in veterinary medicine, ivermectin is used to treat billions of livestock and pets around the world, helping to boost production of food and leather products, as well as to keep billions of companion animals, particularly dogs and horses, healthy. Nowadays, ivermectin by its own has produced sales greater than US$1 billion\/annum during the past two decades [<a id=\"__tag_670054643\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>] and is annually taken by close to 250 million people [<a id=\"__tag_670054552\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>].<\/p>\n<p id=\"__p3\">Ivermectin belongs to the group of avermectins (AVM), which is a group of 16-membered macrocyclic lactone compounds discovered in 1967 in the Japanese Kitasato Institute [<a id=\"__tag_670054614\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>,<a id=\"__tag_670054567\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>] in fermentation broths of actinomycetes cultures with the fungus\u00a0<em>Streptomyces avermitilis<\/em>\u00a0[<a id=\"__tag_670054574\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b4\" aria-expanded=\"false\" aria-haspopup=\"true\">4<\/a>&#8211;<a id=\"__tag_670054564\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b6\" aria-expanded=\"false\" aria-haspopup=\"true\">6<\/a>]. AVM family members include, among others, selamectin, abamectin, monoxidectin and ivermectin (<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/figure\/fig01\/\" target=\"figure\" rel=\"noopener\">Figure 1<\/a>), all of which differ from the antibacterial and antifungal 16-membered macrocyclic lactones by owning a bisoleandrosyloxy substituent at the C13 [<a id=\"__tag_670054605\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>]. Ivermectin is the most commonly employed compound from the AVM group, being a more potent and safer semi-synthetic mixture of the two AVMs 22,23-dihydroavermectin-B<sub>1a<\/sub>\u00a0and dihydroavermectin-B<sub>1b<\/sub>, at a reason of 4:1, respectively [<a id=\"__tag_670054634\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>]. Since 1981 ivermectin has been employed for agriculture, veterinary and aquaculture purposes [<a id=\"__tag_670054624\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>], and is recognized as nematocidal, acaracidal and insecticidal [<a id=\"__tag_670054590\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>,<a id=\"__tag_670054607\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]. The antiparasitic efficacy of ivermectin is not limited to onchocerciasis, since it is also effective for filarial infections such as those caused by\u00a0<em>Wuchereria bancrofti, Brugia malayi, Loa loa, Mansonella perstans,<\/em>\u00a0and\u00a0<em>Mansonella ozzardi<\/em>\u00a0[<a id=\"__tag_670054560\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]. It also eradicates gastrointestinal parasites, including\u00a0<em>Ascaris lumbricoides, Strongyloides stercoralis, Enterobius vermicularis, Trichuris trichiuria,<\/em>\u00a0and\u00a0<em>Ancylostoma duodenale<\/em>\u00a0[<a id=\"__tag_670054594\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]. Besides, it is employed to treat trypanosomiasis, malaria, leishmaniasis, scabies and head lice [<a id=\"__tag_670054622\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>]. In parasites and helminths, ivermectin, as well as the rest of AVMs, increases the activity of \u03b3-aminobutyric acid (GABA) receptors or glutamate-gated chloride ion channels (Glu-Cl) [<a id=\"__tag_670054571\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>,<a id=\"__tag_670054549\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b4\" aria-expanded=\"false\" aria-haspopup=\"true\">4<\/a>,<a id=\"__tag_670054548\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b6\" aria-expanded=\"false\" aria-haspopup=\"true\">6<\/a>], which blockades the signal between neuron and muscle [<a id=\"__tag_670054563\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>].<\/p>\n<div id=\"fig01\" class=\"fig iconblock whole_rhythm\">\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140616663864784\"><img decoding=\"async\" class=\"fig-image\" title=\"An external file that holds a picture, illustration, etc. Object name is ajcr0008-0317-f1.jpg\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/bin\/ajcr0008-0317-f1.jpg\" alt=\"An external file that holds a picture, illustration, etc. Object name is ajcr0008-0317-f1.jpg\" \/><\/div>\n<div id=\"lgnd_fig01\" class=\"icnblk_cntnt\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/figure\/fig01\/\" target=\"figure\" rel=\"noopener\">Figure 1<\/a><\/div>\n<div class=\"caption\">\n<p id=\"__p5\">Compounds belonging to the avermectin family. Some AVM family members are showed in the picture. All of them are recognized by a 16-carbon macrocyclic lactone core with the bisoleandrosyloxy substituent at C13. The characteristics substituents of ivermectin are marked in gray.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<p id=\"__p4\" class=\"p\">In mammals, GABA-sensitive neurons are secured by the blood-brain barrier (BBB) within the central nervous system (CNS), protecting vertebrates against potential harmful effects of AVMs [<a id=\"__tag_670054593\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>,<a id=\"__tag_670054586\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b6\" aria-expanded=\"false\" aria-haspopup=\"true\">6<\/a>]. In support of this statement, subpopulation of collie dogs that have defective function of the multidrug resistance (MDR) protein (commonly a 4 base-pair deletion of the\u00a0<em>mdr-1<\/em>\u00a0gene which produces a stop codon), which is an integral part of the BBB and functions as a drug-transport pump in the BBB, have increased neurotoxicity to ivermectin [<a id=\"__tag_670054631\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b8\" aria-expanded=\"false\" aria-haspopup=\"true\">8<\/a>]. On the other hand, invertebrates are dose-dependent susceptible due to the widespread allocation of Glu-Cl channels, in whom ivermectin induces the opening of GABA-regulated Cl- channels that generates an influx of Cl- [<a id=\"__tag_670054654\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>,<a id=\"__tag_670054637\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]. The resulting hyperpolarization impedes the phosphorylation of the regulatory light chain of myosin II by PAK1 [<a id=\"__tag_670054602\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b9\" aria-expanded=\"false\" aria-haspopup=\"true\">9<\/a>], promoting paralysis of somatic muscles with concomitant uncoordinated movement, starvation due to inhibition of pharyngeal pumping, and death [<a id=\"__tag_670054561\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>,<a id=\"__tag_670054579\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b3\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>,<a id=\"__tag_670054652\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b5\" aria-expanded=\"false\" aria-haspopup=\"true\">5<\/a>,<a id=\"__tag_670054638\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]. Certainly, the affinity of ivermectin for the parasite is 100 times greater than for the mammalian brain, but at least in onchocerciasis, ivermectin action is mostly restricted to the microfilariae stage of\u00a0<em>Onchocerca volvulus<\/em>\u00a0[<a id=\"__tag_670054627\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>] as the macrofilariae form does not require pharyngeal pumping to survive [<a id=\"__tag_670054608\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>]. The rate of reduction in microfilarial worms is close to 98% with only two weeks of ivermectin administration, an effect maintained within the next 12 months [<a id=\"__tag_670054577\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>].<\/p>\n<\/div>\n<div id=\"__sec2\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec2title\" class=\"head no_bottom_margin ui-helper-clearfix\">Current use and dosage as an antiparasitic drug<\/h2>\n<p id=\"__p6\" class=\"p p-first-last\">In humans, the most used dose of ivermectin for onchocerciasis, strongyloidiasis and enterobiasis ranges between 150 to 200 \u00b5g\/kg [<a id=\"__tag_670054615\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b10\" aria-expanded=\"false\" aria-haspopup=\"true\">10<\/a>&#8211;<a id=\"__tag_670054588\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b12\" aria-expanded=\"false\" aria-haspopup=\"true\">12<\/a>], while it is used at higher doses of 400 \u00b5g\/kg for lymphatic filariasis [<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b13\" aria-expanded=\"false\" aria-haspopup=\"true\">13<\/a>]. It is noteworthy the report of a clinical trial on the use of ivermectin for patients with spinal damage and muscle spasms where the drug was administered up to 1.6 mg\/kg subcutaneously twice a week for 12 weeks [<a id=\"__tag_670389985\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b14\" aria-expanded=\"false\" aria-haspopup=\"true\">14<\/a>].<\/p>\n<\/div>\n<div id=\"__sec3\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec3title\" class=\"head no_bottom_margin ui-helper-clearfix\">Toxicity<\/h2>\n<p id=\"__p7\" class=\"p p-first\">This compound has a wide margin of safety in ruminants, pigs and equine, as well as in most of the dog breeds [<a id=\"__tag_670054655\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b15\" aria-expanded=\"false\" aria-haspopup=\"true\">15<\/a>,<a id=\"__tag_670054543\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b16\" aria-expanded=\"false\" aria-haspopup=\"true\">16<\/a>]. The acute toxicity of ivermectin has been investigated in various species of animals. The signs of toxicity were similar after oral and intraperitoneal administration in rats and mice, and the effects consisted in ataxia, tremors, and reduced activity [<a id=\"__tag_670054568\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b17\" aria-expanded=\"false\" aria-haspopup=\"true\">17<\/a>]. In early stages of development, ivermectin at doses of 0.4-0.8 mg\/kg in mice, 10 mg\/kg in rats, and 3-6 mg\/kg in rabbits, increased the incidence of cleft palate, but it was not considered as embryotoxic since the frequency of anomalies was very low [<a id=\"__tag_670054619\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b18\" aria-expanded=\"false\" aria-haspopup=\"true\">18<\/a>]. The toxic effects have been related to its interaction with the P-glycoprotein, which limits its access to the CNS. The absence of this protein determines the accumulation of ivermectin in the brain of transgenic mice that do not express it. Finally, in adult Rhesus monkeys that ingested it daily for 16 days at 1.2 mg\/kg, no undesirable effects were detected [<a id=\"__tag_670054578\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b18\" aria-expanded=\"false\" aria-haspopup=\"true\">18<\/a>].<\/p>\n<p id=\"__p8\">There are several toxicological reports of ivermectin in different species. The lethal dose 50 (LD50) reported in mice [<a id=\"__tag_670054553\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b19\" aria-expanded=\"false\" aria-haspopup=\"true\">19<\/a>] is 25 mg\/kg administered orally, whose human equivalent dose (HED) is 2.02 mg\/kg. The LD50 increases up to 30 mg\/kg when this compound is administered intraperitoneally in mice (HED 2.43 mg\/kg). For rats the average lethal dose is 50 mg\/kg orally (HED 8.01 mg\/kg) and 55 mg\/kg intraperitoneally (HED 8.91 mg\/kg). In rabbits it is 406 mg\/kg in topical application, while in dogs it is 80 mg\/kg administered orally (HED 43.24 mg\/kg) [<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b20\" aria-expanded=\"false\" aria-haspopup=\"true\">20<\/a>]. Clearly, it seems that the higher the phylogenetic scale the lower toxicity by ivermectin. These data are in accord with the findings in a review paper on avermectins poisoning (14 on suicidal attempt). In this retrospective review, among 18 patients exposed to abamectin and one to ivermectin, 15 were poisoned by oral ingestion. Four were asymptomatic and 8 had minor symptoms with a mean ingestion of 23 mg\/kg (range in 4.2-67 mg\/kg). Seven patients manifested severe symptoms, such as coma (seven), aspiration with respiratory failure (four), and hypotension (three), after a mean ingestion of 100.7 mg\/kg avermectin (15.4 mg\/kg for ivermectin and 114.9 mg\/kg for abamectin). All 7 seven patients received intensive supportive care; 1 patient died 18 days later as a result of multiple organ failure [<a id=\"__tag_670054603\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b21\" aria-expanded=\"false\" aria-haspopup=\"true\">21<\/a>].<\/p>\n<p id=\"__p9\">In humans it is considered that ivermectin generates low levels of toxicity because its targets are confined within the CNS. Indeed, most patients treated with ivermectin have no side-effects other than those caused by the immune and inflammatory responses against the parasite, such as fever, pruritus, skin rashes and malaise [<a id=\"__tag_670054589\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>,<a id=\"__tag_670054650\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b22\" aria-expanded=\"false\" aria-haspopup=\"true\">22<\/a>], and when present, they appear within 24-48 h after treatment [<a id=\"__tag_670054612\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b23\" aria-expanded=\"false\" aria-haspopup=\"true\">23<\/a>]. Certainly, moderate symptoms such as arthralgia, dizziness, fever, skin edema, dyspnea and hypotension may be more related with the microfilarial load in the patient rather than with the intrinsic toxicity of ivermectin [<a id=\"__tag_670389986\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b24\" aria-expanded=\"false\" aria-haspopup=\"true\">24<\/a>]. Reports on cases of encephalopathy in patients co-infected with onchocerciasis and lymphatic filariasis after 48 h of treatment with ivermectin can be found in the literature [<a id=\"__tag_670054570\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b25\" aria-expanded=\"false\" aria-haspopup=\"true\">25<\/a>], but it is believed that this adverse reaction is due to the obstruction of the microcirculation of the brain by the accumulation of dead or paralyzed parasites, which leads to brain embolism [<a id=\"__tag_670054629\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b26\" aria-expanded=\"false\" aria-haspopup=\"true\">26<\/a>].<\/p>\n<p id=\"__p10\" class=\"p p-last\">In conclusion, the immense number of patients who have been treated with ivermectin shows that it is a safe and a well-tolerated drug. Beyond the side effects attributable to the immunological and inflammatory reaction elicited by dying or death parasites, there are sympathetic signs related to ivermectin intoxication, including tremors, mydriasis, sialorrhea, motor incoordination and coma [<a id=\"__tag_670054662\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b27\" aria-expanded=\"false\" aria-haspopup=\"true\">27<\/a>].<\/p>\n<\/div>\n<div id=\"__sec4\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec4title\" class=\"head no_bottom_margin ui-helper-clearfix\">Pharmacokinetics<\/h2>\n<p id=\"__p11\" class=\"p p-first\">The pharmacokinetics of ivermectin have been widely studied in various mammals, including humans; is a fat-soluble compound, with a distribution volumen of 46.9 L; it has a mean peak plasma level of ~4 h after oral administration with a second peak at 6-12 h because of enterohepatic recycling [<a id=\"__tag_670054657\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>], and possesses an oral clearance of 1.2 L\/h [<a id=\"__tag_670054653\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]. With a plasma protein binding of 93% [<a id=\"__tag_670054559\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b7\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>], this drug experiences low biotransformation within the organism [<a id=\"__tag_670054583\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b4\" aria-expanded=\"false\" aria-haspopup=\"true\">4<\/a>&#8211;<a id=\"__tag_670054635\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b6\" aria-expanded=\"false\" aria-haspopup=\"true\">6<\/a>]. The maximum concentration in plasma is reached 4-5 h after its oral administration; its half-life is approximately 19 h and is metabolized in the liver by the cytochrome CYP1A and CYP3A4 complexes, generating 10 metabolites, mostly demethylated and hydroxylated. Its excretion is mainly by feces and only 1% is excreted in the urine [<a id=\"__tag_670054668\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b28\" aria-expanded=\"false\" aria-haspopup=\"true\">28<\/a>].\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl01\/\" target=\"table\" rel=\"noopener\">Table 1<\/a>\u00a0shows the pharmacokinetic data of ivermectin in humans infected with parasites, as well as in healthy humans treated with various doses of ivermectin [<a id=\"__tag_670054632\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b28\" aria-expanded=\"false\" aria-haspopup=\"true\">28<\/a>,<a id=\"__tag_670054566\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b29\" aria-expanded=\"false\" aria-haspopup=\"true\">29<\/a>]. According with\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl01\/\" target=\"table\" rel=\"noopener\">Table 1<\/a>, the molar concentrations achieved taking into account the total exposure of the drug measured by the area under the curve (systemic exposure) in healthy individuals or patients treated for parasitic diseases are:<\/p>\n<div id=\"tbl01\" class=\"table-wrap anchored whole_rhythm\">\n<h3>Table 1<\/h3>\n<div class=\"caption\">\n<p id=\"__p13\">Pharmacokinetics of ivermectin in healthy and parasited subjects<\/p>\n<\/div>\n<div class=\"xtable\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140616584165200\">\n<table class=\"rendered small default_table\" frame=\"hsides\" rules=\"groups\" cellspacing=\"3\" cellpadding=\"2\">\n<thead>\n<tr>\n<th colspan=\"1\" rowspan=\"1\" align=\"left\">Condition<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Doses (mg\/kg)<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Route<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">C<sub>max<\/sub>\u00a0(ng\/mL)<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">T<sub>max<\/sub>\u00a0(h)<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">AUC (\u00b5g\/h\/mL)<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Parasitic infection<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.1-0.2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Oral<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">52.0<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5.2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">2.852<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Healthy<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.35-0.6<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Oral<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">87.0<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">4.2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1.444<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Healthy<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.7-1.1<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Oral<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">165.2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">3.6<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">2.099<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Healthy<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1.4-2.0<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Oral<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">247.8<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">4.2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">4.547<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<p id=\"__p12\" class=\"p\">3.25 \u03bcM\/h in parasitized patients with a dose of 0.1-0.2 mg\/kg, 1.64 \u03bcM in healthy subjects with a dose of 0.35-0.6 mg\/kg, 2.4 \u03bcM\/h in healthy subjects with a dose of 0.7-1.1 mg\/kg, and 5.2 \u03bcM\/h in healthy subjects with a dose of 2 mg\/kg.<\/p>\n<\/div>\n<div id=\"__sec5\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec5title\" class=\"head no_bottom_margin ui-helper-clearfix\">Drug repurposing in cancer<\/h2>\n<p id=\"__p14\" class=\"p p-first\">Drug repurposing, drug redirecting or drug reprofiling is defined as the identification of novel usages for existing drugs. Both development risks and costs, as well as safety-related failure, are reduced with this approach because such drugs have well-known formulation development,\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0screening, as well as pharmacokinetic and pharmacodynamic profiles. Also, the first clinical phases of many drugs had been completed and can be bypassed to reduce several years of development. Therefore, drug repurposing has the potential to reduce the whole process up to 3-12 years, and consequently, the potential recycle of compounds towards a new indication is an attractive opportunity for patients on need [<a id=\"__tag_670054597\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b30\" aria-expanded=\"false\" aria-haspopup=\"true\">30<\/a>]. The relevance of drug repositioning in research is demonstrated with the fact that since the first publication of the subject back to 2004, there are more than 500 papers about it until 2013, at least in PubMed [<a id=\"__tag_670054576\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b31\" aria-expanded=\"false\" aria-haspopup=\"true\">31<\/a>]. However, the majority of repositioned agents were discovered before starting with systematic efforts on 2006 to identify drugs with potential additional use, suggesting a serendipitous detection [<a id=\"__tag_670054551\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b30\" aria-expanded=\"false\" aria-haspopup=\"true\">30<\/a>,<a id=\"__tag_670054641\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b32\" aria-expanded=\"false\" aria-haspopup=\"true\">32<\/a>,<a id=\"__tag_670054587\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b33\" aria-expanded=\"false\" aria-haspopup=\"true\">33<\/a>]. Currently, other ways to identify compounds with repositioning potential are informed insights and platforms established to identify\u00a0<em>in silico<\/em>\u00a0repositioning opportunities [<a id=\"__tag_670054542\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b32\" aria-expanded=\"false\" aria-haspopup=\"true\">32<\/a>,<a id=\"__tag_670054540\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b34\" aria-expanded=\"false\" aria-haspopup=\"true\">34<\/a>]. Furthermore, with the recently assembly of the Drug Repurposing Hub, an online repurposing library that systematically classifies a collection of clinically tested compounds from existing databases, now it is possible to easily search and view drugs according to their clinical status, drug indications, or mechanism of action, allowing to rapidly find agents for further evaluation [<a id=\"__tag_670054555\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b35\" aria-expanded=\"false\" aria-haspopup=\"true\">35<\/a>].<\/p>\n<p id=\"__p15\" class=\"p p-last\">Important efforts have been made for drug repositioning in cancer. Pantziarka\u00a0<em>et al.<\/em>\u00a0have recently summarized on this topic. They report at least 235 non-cancer drugs with proven antitumor activity either\u00a0<em>in vitro<\/em>\u00a0or\u00a0<em>in vivo<\/em>, and among these, 67 (29%) are in the World Health Organization (WHO) list of essential medicines, and 176 (75%) are off-patent [<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b36\" aria-expanded=\"false\" aria-haspopup=\"true\">36<\/a>]. 133 (57%) had human data in cancer patients [<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b36\" aria-expanded=\"false\" aria-haspopup=\"true\">36<\/a>]. Four were listed in clinical guidelines, namely thalidomide, all-trans retinoic acid, zoledronic acid and non-steroidal anti-inflammatory drugs (NSAID) [<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b36\" aria-expanded=\"false\" aria-haspopup=\"true\">36<\/a>]. Of note, at least 3 drugs have shown a survival benefit in randomized trials: cimetidine (colorectal cancer), progesterone (breast cancer) and itraconazole (lung cancer) [<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b36\" aria-expanded=\"false\" aria-haspopup=\"true\">36<\/a>]. Few examples of drug-target networks analyses show that both simvastatin and ketoconazole are anti-proliferative compounds in breast cancer [<a id=\"__tag_670054581\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b32\" aria-expanded=\"false\" aria-haspopup=\"true\">32<\/a>], while gene expression profiles suggest that topiramate can be used to treat small-cell lung cancer and that sirolimus can be useful for glucocorticoid-resistant acute lymphocytic leukemia [<a id=\"__tag_670054584\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b35\" aria-expanded=\"false\" aria-haspopup=\"true\">35<\/a>]. On the other hand, drugs that were first formulated to treat cancer might also be useful to treat non-malignant diseases. That is the case of inhibitors of histone deacetylase enzymes that are approved for T-cell lymphoma, but are prospective targets for malaria, leishmaniasis and trypanosomiasis [<a id=\"__tag_670054550\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b37\" aria-expanded=\"false\" aria-haspopup=\"true\">37<\/a>].<\/p>\n<\/div>\n<div id=\"__sec6\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec6title\" class=\"head no_bottom_margin ui-helper-clearfix\">Molecular mechanisms of the antitumor effects of ivermectin<\/h2>\n<p id=\"__p16\" class=\"p p-first\">Despite the relatively short time on which ivermectin has been investigated as a drug for cancer repositioning, a number of molecular mechanisms of action have been discovered (<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/figure\/fig02\/\" target=\"figure\" rel=\"noopener\">Figure 2<\/a>\u00a0and\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl02\/\" target=\"table\" rel=\"noopener\">Table 2<\/a>). Among these are the following:<\/p>\n<div id=\"fig02\" class=\"fig iconblock whole_rhythm\">\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140616623679440\"><img decoding=\"async\" class=\"fig-image\" title=\"An external file that holds a picture, illustration, etc. Object name is ajcr0008-0317-f2.jpg\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/bin\/ajcr0008-0317-f2.jpg\" alt=\"An external file that holds a picture, illustration, etc. Object name is ajcr0008-0317-f2.jpg\" \/><\/div>\n<div id=\"lgnd_fig02\" class=\"icnblk_cntnt\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/figure\/fig02\/\" target=\"figure\" rel=\"noopener\">Figure 2<\/a><\/div>\n<div class=\"caption\">\n<p id=\"__p17\">Antitumor mechanisms of ivermectin. 1. Ivermectin inhibits the P-glycoprotein pump, that induces a multidrug phenotype in the cancer cell. 2. Ivermectin acts as an ionophore and up-regulates chloride channels to generate apoptosis and osmotic cell death. 3. By decreasing the function of the mitochondrial complex I, ivermectin limits the electronic movement in the oxidative phosphorylation pathway that stimulates oxygen consumption rate to generate ATP for the cell. Concomitantly there is a reduction in the phosphorylation levels of Akt, impacting in the mitochondrial biogenesis process. Furthermore, alterations in the mitochondrial machinery are related with increased levels of reactive oxygen species that damage DNA. 4. Ivermectin induces ICD through the stimulation of an ATP- and HMGB1-enriched microenvironment, which promotes inflammation. This drug also increases ATP sensitivity and calcium signals in P2X membranal receptors, particularly P2X4 and P2X7, to induce ATP-dependent immune responses. 5. Ivermectin promotes the poly-ubiquitination of the kinase PAK1, which directs it to degradation in the proteasome. Defective PAK1, in turn, inhibits the Akt\/mTOR pathway. At the same time, ivermectin stimulates the expression of Beclin1 and Atg5, both related with induction of autophagy and reduces the function of the negative regulator of apoptosis Bcl-2. Together, this generates autophagy and apoptosis. 6. Ivermectin represses AXIN2, LGR5 and ASCL2, all of them positive regulators of WNT-TCF while promotes the repressor of the WNT signaling FILIP1L. Concomitantly, ivermectin promotes the expression of several IFN-related genes, such as\u00a0<em>IFIT1, IFIT2, IF144, ISG20, IRF9<\/em>\u00a0and\u00a0<em>OASL<\/em>. 7. Ivermectin modifies the epigenetic signature and the self-renewal activity in the malignant cell due to its ability to mimic the SIN3-interaction that binds to the PAH2 motif of the cancer-associated deregulators SIN3A and SIN3B. SIN3A naturally induces NANOG and SOX2, which are stimulants of stem cell pluripotency. 8. Ivermectin limits the function of the RNA helicases NS3 and DDX23, both of which are related with ribosome biogenesis and post-transcriptional modifications, as well as with mRNA degradation. DDX23 acts as a promoter of miR-21, which is a well-recognized stimulator of tumor progression. 9. Ivermectin inhibitis preferentially the CSC population and up-regulates pluripotency and self-renewal genes NANOG, SOX2 and OCT4. IVM: ivermectin; ATP: adenosine triphosphate; OCR: oxygen consumption rate; ROS: reactive oxygen species.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"tbl02\" class=\"table-wrap anchored whole_rhythm\">\n<h3>Table 2<\/h3>\n<div class=\"caption\">\n<p id=\"__p19\">Summary of the antitumor targets of ivermectin<\/p>\n<\/div>\n<div class=\"xtable\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140616583837376\">\n<table class=\"rendered small default_table\" frame=\"hsides\" rules=\"groups\" cellspacing=\"3\" cellpadding=\"2\">\n<thead>\n<tr>\n<th colspan=\"1\" rowspan=\"1\" align=\"left\">Target<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Effect<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">References<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">MDR protein<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Inhibition<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b39\" aria-expanded=\"false\" aria-haspopup=\"true\">39<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Chloride channel<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Increase of activity<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b44\" aria-expanded=\"false\" aria-haspopup=\"true\">44<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Akt\/mTOR pathway<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Inhibition<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b47\" aria-expanded=\"false\" aria-haspopup=\"true\">47<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">P2X7\/P2X7 receptors<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Activation<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b50\" aria-expanded=\"false\" aria-haspopup=\"true\">50<\/a>,<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b51\" aria-expanded=\"false\" aria-haspopup=\"true\">51<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">PAK1 protein<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Inhibition<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b9\" aria-expanded=\"false\" aria-haspopup=\"true\">9<\/a>,<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b54\" aria-expanded=\"false\" aria-haspopup=\"true\">54<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">WNT-TCF pathway<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Inhibition<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>,<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b58\" aria-expanded=\"false\" aria-haspopup=\"true\">58<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">SIN3 domain<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Inhibition<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b59\" aria-expanded=\"false\" aria-haspopup=\"true\">59<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">NS3 DDX23 helicase<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Inhibition<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b64\" aria-expanded=\"false\" aria-haspopup=\"true\">64<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><em>Nanog\/Sox2\/Oct4<\/em>\u00a0genes<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Downregulation<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b67\" aria-expanded=\"false\" aria-haspopup=\"true\">67<\/a>]<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<div id=\"__sec7\" class=\"sec\">\n<h3 id=\"__sec7title\">Ivermectin as an inhibitor of the multi-drug resistance (MDR) phenotype<\/h3>\n<p id=\"__p18\" class=\"p p-first\">The firsts reports on the potential antitumor activity of ivermectin appeared almost 20 years ago, and these were somehow linked with the recently discovered basis for the MDR phenotype at that time. Gros P\u00a0<em>et al.<\/em>\u00a0in 1986 reported the isolation of DNA clones complementary to the cellular messenger RNA transcripts of\u00a0<em>MDR<\/em>\u00a0genes, and showed that high-level expression of a full-length complementary DNA clone in an otherwise drug-sensitive cell confers a complete MDR phenotype [<a id=\"__tag_670054592\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b38\" aria-expanded=\"false\" aria-haspopup=\"true\">38<\/a>]. Later on, Didier and Loor proved by a short-term assay the P-glycoprotein function inhibition, which measures the restoration of the retention of two P-glycoprotein probes in MDR cells to their parental cells, concluding that ivermectin is also a substrate and an inhibitor of P-glycoprotein [<a id=\"__tag_670054648\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b39\" aria-expanded=\"false\" aria-haspopup=\"true\">39<\/a>]. Though not named as ivermectin, avermectin IB1 showed the ability to reduce tumor growth\u00a0<em>in vivo<\/em>\u00a0by 50% at day 5 at dose of 1 mg\/kg (HED 81 \u03bcg\/kg) in SHK male mice bearing a solid Ehrlich carcinoma. In addition, at the same dose it inhibits the growth of the carcinoma cell line 755 (C57\/BL6 male mice), and the tumor growth inhibition value reached a maximum when avermectin B1 was injected on day 3 after tumor inoculation. Based on the fact that ivermectin inhibits multidrug resistance in tumors, avermectin IB1 was tested with vincristine in the Ehrlich carcinoma, with results indicating that the antitumor effect of vincristine is greatly increased when avermectin 1B1 is administered after vincristine [<a id=\"__tag_670054618\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b40\" aria-expanded=\"false\" aria-haspopup=\"true\">40<\/a>]. No further studies have exploited the anti-MDR effect of ivermectin. Nevertheless, the search for novel strategies and\/or schedule optimization of MDR inhibitors continues [<a id=\"__tag_670054625\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b41\" aria-expanded=\"false\" aria-haspopup=\"true\">41<\/a>], which suggests that there is still room for investigating ivermectin roles in reversing or preventing the development of the MDR phenotype.<\/p>\n<\/div>\n<div id=\"__sec8\" class=\"sec\">\n<h3 id=\"__sec8title\">Ivermectin as an ionophore drug<\/h3>\n<p id=\"__p20\" class=\"p p-first\">The term \u2018ionophore\u2019 was first used in 1967 in reference of the ability of organic molecules to bind metal cations and to form lipid soluble complexes that facilitate their transport across cellular membranes. Thus, ionophores can diffuse back and forth between the extracellular and intracellular spaces, or may remain in the plasma membrane as their transport metal ions between intracellular and extracellular spaces [<a id=\"__tag_670054644\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b42\" aria-expanded=\"false\" aria-haspopup=\"true\">42<\/a>]. Ionophore antibiotics act by generating pores in biological membranes that dramatically alter the ionic household of cells. Salinomycin is an example of an ionophore antibiotic which generates ion channel-like structures that exhibit strong selectivity for K<sup>+<\/sup>, but other monovalent cations are also conducted (e.g., Na<sup>+<\/sup>\u00a0and H<sup>+<\/sup>) [<a id=\"__tag_670054565\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b43\" aria-expanded=\"false\" aria-haspopup=\"true\">43<\/a>]. Traditionally, the cell-killing activity of ionophore antibiotics is thought to originate from profoundly deregulating osmosis, as well as from direct cytotoxic effects of the altered biochemical landscape. It is known that malignant cells tend to upregulate chloride channels, which potentially could mark them as more sensitive to alterations in chloride flux, and that an unbalance in intracellular chloride concentrations affects intracellular Ca<sup>2+<\/sup>\u00a0levels, as well as pH and cell volume, which can lead to apoptosis in the affected cell [<a id=\"__tag_670054557\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b44\" aria-expanded=\"false\" aria-haspopup=\"true\">44<\/a>].<\/p>\n<p id=\"__p21\" class=\"p p-last\">In line with these statements, in a screen of a small chemical library of antibiotics and metabolic regulators to identify anti-leukemia compounds, Sharmeen\u00a0<em>et al.<\/em>\u00a0found that ivermectin induces cell death at low micromolar concentrations (IC50 of 10 \u03bcM) in HL60, KG1a, and OCI-AML2 acute myeloid leukemia cell lines, as well as in primary patient samples. Cell death was caspase-dependent and interestingly, normal hematopoietic cells were much less sensitive to ivermectin as it did not induce apoptosis at concentrations up to 20 \u03bcM. Similar effects were seen when leukemia and normal cells were tested for clonogenicity. They also showed that increased chloride influx correlated with cell death and changes in both cell size and cell hyperpolarization, as these effects were much more marked in sensitive leukemia cells as compared to normal cells. Interestingly, there was a synergistic or additive interaction in OCI-AML2 and U937, but not in normal cells, when they treated with ivermectin plus cytarabine or daunorubicin [<a id=\"__tag_670054636\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b44\" aria-expanded=\"false\" aria-haspopup=\"true\">44<\/a>].<\/p>\n<\/div>\n<div id=\"__sec9\" class=\"sec\">\n<h3 id=\"__sec9title\">Ivermectin as an inductor of mitochondrial dysfunction and oxidative damage<\/h3>\n<p id=\"__p22\" class=\"p p-first-last\">On the basis that anthelmintics or antibiotics may target mitochondria in mammalian cells [<a id=\"__tag_670054664\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b45\" aria-expanded=\"false\" aria-haspopup=\"true\">45<\/a>,<a id=\"__tag_670389984\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b46\" aria-expanded=\"false\" aria-haspopup=\"true\">46<\/a>], ivermectin was tested in glioblastoma cell lines to identify whether its antitumor effect occurs via inhibition of mitochondrial biogenesis or function. As expected, ivermectin inhibits in a dose-dependent manner the basal and maximum oxygen consumption rate (OCR), most likely by decreasing the enzyme activity of respiratory complex I but not II, IV or V, and consistent with that, both the membrane potential and electrochemical proton gradient decrease while a significant increase in mitochondrial superoxide and decreased ATP are observed. Furthermore, by establishing a subline of the U87 cell line deficient in mitochondrial respiration it was proved that under these conditions ivermectin was unable to induce cell death, as well as when these cells were co-treated with the antioxidants alfa-tocopherol or mannitol. These effects were tracked down by studying the Akt\/mTOR pathway, which at least in part controls mitochondria biogenesis and function. Results showed that ivermectin decreases phosphorylation of Akt (S473), mTOR (S2481) and the ribosomal S6 protein (rS6) in U87, T98G and HBMEC cells, indicating that ivermectin inhibits the Akt\/mTOR pathway [<a id=\"__tag_670054596\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b47\" aria-expanded=\"false\" aria-haspopup=\"true\">47<\/a>]. The effect of ivermectin upon mitochondria and oxidative damage has been recently corroborated in a study on renal cancer cell lines, where it was demonstrated that renal cancer cells do have higher mitochondrial mass and basal and maximal OCR [<a id=\"__tag_670054545\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b48\" aria-expanded=\"false\" aria-haspopup=\"true\">48<\/a>]. As in the Liu\u00a0<em>et al.<\/em>\u00a0study, ivermectin in this model also decreased mitochondrial membrane potential as well as basal and maximal respiratory capacities. Of note, ivermectin significantly increased intracellular ROS and 8-OHdG levels, suggesting that the antitumoral effects of ivermectin are related to oxidative stress and DNA damage. This was confirmed by the abolition of the inhibitory effect of ivermectin in these renal cancer cell lines when co-treated with acetyl-L-carnitine (ALCAR) or N-acetyl-L-cysteine (NAC), a stimulant of mitochondrial biogenesis and an antioxidant, respectively [<a id=\"__tag_670054580\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b48\" aria-expanded=\"false\" aria-haspopup=\"true\">48<\/a>].<\/p>\n<\/div>\n<div id=\"__sec10\" class=\"sec\">\n<h3 id=\"__sec10title\">Ivermectin as an inductor of immunogenic cell death (ICD)<\/h3>\n<p id=\"__p23\" class=\"p p-first-last\">Immunogenic cell death (ICD) is characterized by the presence of damage-associated molecular patterns (DAMPs) such as the membranal exposure of calreticulin and the release of both ATP and high-mobility group box 1 (HMGB1) into the extracellular space, which are then recognized by immune cells to elicit antineoplastic activities [<a id=\"__tag_670054600\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b49\" aria-expanded=\"false\" aria-haspopup=\"true\">49<\/a>]. Exogenous ATP regulates defense through P1, P2X and P2Y purinergic receptors [<a id=\"__tag_670054669\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b50\" aria-expanded=\"false\" aria-haspopup=\"true\">50<\/a>]. However, recently P2X7 overexpression has been correlated with promotion of both tumor growth and metastases [<a id=\"__tag_670054611\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b50\" aria-expanded=\"false\" aria-haspopup=\"true\">50<\/a>]. Although within the tumor microenvironment ATPases such CD39 and CD73 degrade ATP to its immunosuppressive form, adenosine, ivermectin can surpass their effect by a potent induction of both HMGB1 and ATP extrusion which, in turn, induces inflammation [<a id=\"__tag_670054572\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b50\" aria-expanded=\"false\" aria-haspopup=\"true\">50<\/a>]. Certainly, it has been reported in triple-negative breast cancer cells (TNCB) that ivermectin allosterically potentiates P2X4\/P2X7- and caspase-1-mediated ICD due to the stimulation of an ATP-enriched tumor microenvironment, disrupting the balance between the survival and cytotoxic roles of purinergic signaling in malignant cells, which also induces autophagy [<a id=\"__tag_670054663\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b50\" aria-expanded=\"false\" aria-haspopup=\"true\">50<\/a>]. One additional work done with human monocyte-derived macrophages corroborated the ivermectin association with PX24 and P2X7 receptors. Such research demonstrated that ivermectin increases ATP sensitivity and delays current deactivation after ATP wash-out in PX24 receptors, which together with the augmentation of ATP-induced currents and Ca<sup>2+<\/sup>\u00a0signals in P2X7 receptors, suggests that ivermectin may stimulate ATP-dependent immune responses [<a id=\"__tag_670054626\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b51\" aria-expanded=\"false\" aria-haspopup=\"true\">51<\/a>]. Altogether, literature indicates that ivermectin could promote and potentiate ICD at the tumor microenvironment.<\/p>\n<\/div>\n<div id=\"__sec11\" class=\"sec\">\n<h3 id=\"__sec11title\">Ivermectin as an inductor of autophagy<\/h3>\n<p id=\"__p24\" class=\"p p-first-last\">Autophagy, a self-degradative catabolic pathway is characterized by formation of doublemembrane autophagosomes, which sequester excess or defective organelles and fuse with lysosomes for degradation of enclosed materials in the lysosome to mobilize energy and nutrients under certain cellular stimuli such as starvation, developmental transitions, hypoxia and\/or oxidative stress [<a id=\"__tag_670054646\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b52\" aria-expanded=\"false\" aria-haspopup=\"true\">52<\/a>,<a id=\"__tag_670054645\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b53\" aria-expanded=\"false\" aria-haspopup=\"true\">53<\/a>]. Ivermectin in ovarian and glioblastoma cancer cell lines promotes ubiquitination-mediated degradation of the oncogenic kinase PAK1 [<a id=\"__tag_670054640\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>,<a id=\"__tag_670054649\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b47\" aria-expanded=\"false\" aria-haspopup=\"true\">47<\/a>], a key protein in cytoskeletal reorganization and nuclear signaling for tumor growing in more than 70% of all human cancers [<a id=\"__tag_670054547\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>]. PAK1 downregulation in turn blockades the repressor of autophagy Akt\/mTOR as evidenced by decreased phosphorylation of Akt, mTOR, p70S6K and 4EBP1 via direct interaction of PAK1 with Akt [<a id=\"__tag_670054651\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b54\" aria-expanded=\"false\" aria-haspopup=\"true\">54<\/a>]. In fact, one work with multiple breast cancer cell lines treated with ivermectin showed its autophagy-promoter role by the formation of acidic vesicular organelles, with double-membraned autophagosomes by transmission electronic microscopy, and with the promotion of the expression levels of the autophagy-related proteins Beclin 1 and Atg5 in a dose-dependent fashion. Ivermectin, in turn, increases the interaction of Beclin 1 with positive regulators of autophagy, specifically Atg14L and Vps34, while diminishes its interaction with negative regulators such as Bcl-2 [<a id=\"__tag_670054610\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b54\" aria-expanded=\"false\" aria-haspopup=\"true\">54<\/a>]. All together it is demonstrated that the ivermectin autophagic effects in this model results from inhibition of the PAK1\/Akt\/mTOR pathway.<\/p>\n<\/div>\n<div id=\"__sec12\" class=\"sec\">\n<h3 id=\"__sec12title\">Ivermectin as an inhibitor of the WNT-TCF pathway<\/h3>\n<p id=\"__p25\" class=\"p p-first-last\">The antiproliferative function of ivermectin has been widely documented. A study aimed to find out drug candidates for repositioning with the ability to block the WNT-TCF signaling, which inactivates the tumor suppressor APC in several sporadic human cancers and stimulates the constitutive activation and translocation into the nucleus of \u03b2-catenin during neoplastic transformation [<a id=\"__tag_670054569\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b55\" aria-expanded=\"false\" aria-haspopup=\"true\">55<\/a>,<a id=\"__tag_670054639\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b56\" aria-expanded=\"false\" aria-haspopup=\"true\">56<\/a>], revealed that ivermectin at low micromolar concentrations performs anti-WNT-TCF response in cancer cells [<a id=\"__tag_670054546\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>]. The authors showed the efficacy of ivermectin to inhibit BrdU incorporation in colon cancer, glioblastoma and melanoma cell lines, indicating repression in cell proliferation [<a id=\"__tag_670054604\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>]. Besides, they demonstrated upregulation of activated caspase 3 and repression of the positive WNT-TCF targets\u00a0<em>AXIN2, LGR5,<\/em>\u00a0and\u00a0<em>ASCL2<\/em>\u00a0in DLD1 and Ls174T colon cancer cells [<a id=\"__tag_670054558\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>], opening the possibility to use ivermectin to block WNT-TCF-dependent cancers, such as those from breast, skin, lung and intestine [<a id=\"__tag_670054656\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b1\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>,<a id=\"__tag_670054628\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>]. Moreover, after investigating the ivermectin anti-clonogenic activity by analyzing spheroid formation, they showed that pre-treatment of such cell lines with ivermectin diminishes clonal floating spheroids by up to 73%, which together with the repression of the positive cell cycle regulator cyclin D1, suggests a limiting cancer stem cell formation driven by ivermectin [<a id=\"__tag_670054595\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>]. In fact, ivermectin role as an WNT-TCF inhibitor led to use it as a positive control of such pathway in one research that screened a library of plant and microorganism natural compounds, which confirmed ivermectin suppressor activity by transcriptomic analysis that showed an upregulation of the repressor of WNT signaling\u00a0<em>FILIP1L<\/em>\u00a0up to 10-fold by the use of this compound [<a id=\"__tag_670054621\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b58\" aria-expanded=\"false\" aria-haspopup=\"true\">58<\/a>]. Such study also revealed the increase of the interferon-responsive genes\u00a0<em>ISG20, IFIT1, OASL, IRF9, IF144,<\/em>\u00a0and\u00a0<em>IFIT2<\/em>, in colon cancer cells [<a id=\"__tag_670054601\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b58\" aria-expanded=\"false\" aria-haspopup=\"true\">58<\/a>]. Interestingly, it has been suggested the employment of interferon to inhibit WNT-TCF pathway [<a id=\"__tag_670054544\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b58\" aria-expanded=\"false\" aria-haspopup=\"true\">58<\/a>].<\/p>\n<\/div>\n<div id=\"__sec13\" class=\"sec\">\n<h3 id=\"__sec13title\">Ivermectin as an epigenetic modulator<\/h3>\n<p id=\"__p26\" class=\"p p-first-last\">Other recognized field of action of ivermectin in cancer involves epigenetic regulation. One work done with the breast cancer cell line MDA-MB-231 reported the functional effect of ivermectin after an\u00a0<em>in silico<\/em>\u00a0screen of 2,000 FDA-approved small molecule drugs. The authors evaluated the ability of ivermectin to mimic the SIN3-interaction domain (SID), which naturally binds to the PAH2 motif belonging to the breast cancer-related epigenetic deregulators SIN3A and SIN3B [<a id=\"__tag_670054582\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b59\" aria-expanded=\"false\" aria-haspopup=\"true\">59<\/a>]. By nuclear magnetic resonance they demonstrated that ivermectin indeed blocks the PAH2-SID interaction [<a id=\"__tag_670054667\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b59\" aria-expanded=\"false\" aria-haspopup=\"true\">59<\/a>]. Furthermore, since SIN3A is part of a complex that positively regulates the stem cell and self-renewal markers\u00a0<em>NANOG<\/em>\u00a0and\u00a0<em>SOX2<\/em>, the authors analyzed the role of ivermectin to inhibit such genes and proved that in D3H2LN cells at doses of 0.5 \u00b5M it reduces\u00a0<em>NANOG<\/em>\u00a0and\u00a0<em>SOX2<\/em>\u00a0gene expression by 80%, with a decrease between 90-100% in clonogenic tumorsphere growth [<a id=\"__tag_670054556\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b59\" aria-expanded=\"false\" aria-haspopup=\"true\">59<\/a>].<\/p>\n<\/div>\n<div id=\"__sec14\" class=\"sec\">\n<h3 id=\"__sec14title\">Ivermectin as a helicase inhibitor<\/h3>\n<p id=\"__p27\" class=\"p p-first-last\">RNA helicases represent a large family of proteins implicated in many biological processes, including ribosome biogenesis, splicing, translation and mRNA degradation [<a id=\"__tag_670054630\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b60\" aria-expanded=\"false\" aria-haspopup=\"true\">60<\/a>]. Members of the DEAD-box family of RNA helicases play important roles in various aspects of RNA processing, including transcription, spliceosome biogenesis, ribosome biogenesis, splicing, nucleocytoplasmic transport, translation and decay [<a id=\"__tag_670054575\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b61\" aria-expanded=\"false\" aria-haspopup=\"true\">61<\/a>,<a id=\"__tag_670054660\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b62\" aria-expanded=\"false\" aria-haspopup=\"true\">62<\/a>]. These family members share a conserved core that includes the amino acid sequence D-E-A-D (aspartate-glutamate-alanine-aspartate). They use energy received from ATP hydrolysis to unwind double-stranded RNA, generally act as components of multi-protein complexes, and have diverse functions that depend on their interacting partners. Several DEAD-box RNA helicases are aberrantly expressed in various types of cancer, where they may play important roles in cancer development and\/or progression [<a id=\"__tag_670054665\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b61\" aria-expanded=\"false\" aria-haspopup=\"true\">61<\/a>,<a id=\"__tag_670054613\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b62\" aria-expanded=\"false\" aria-haspopup=\"true\">62<\/a>]. In 2012, Mastrangelo\u00a0<em>et al.<\/em>\u00a0uncovered that ivermectin was an effective inhibitor of the NS3 helicase activity from the Kunjin virus (an Australian variant of the yellow fever virus) by an\u00a0<em>in silico<\/em>\u00a0test, and confirmed it by\u00a0<em>in vitro<\/em>\u00a0helicase enzymatic assays [<a id=\"__tag_670054609\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b63\" aria-expanded=\"false\" aria-haspopup=\"true\">63<\/a>]. On the other hand, a recent study in glioma cell lines has found that the RNA helicase DDX23 regulates the oncogenic miR-21 biogenesis at post-transcriptional level [<a id=\"__tag_670054562\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b64\" aria-expanded=\"false\" aria-haspopup=\"true\">64<\/a>], and it is over-expressed in glioma tissues as compared to normal brain, which is associated with poor survival of glioma patients. After the authors assayed a number of drugs known to inhibit viral helicases, they found that ivermectin inhibits the DDX23-mediated potentiation of pri-miR-21 processing that, in turn, decreases the levels of both precursor and mature miR-21, a well-recognized poor prognostic upregulated marker in cancer [<a id=\"__tag_670054633\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b65\" aria-expanded=\"false\" aria-haspopup=\"true\">65<\/a>]. The treatment with ivermectin in glioma cell lines both\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0was able to induce antitumoral effects, which suggests that the antihelicase role of ivermectin can be considered as another mechanism of its anticancer effect.<\/p>\n<\/div>\n<div id=\"__sec15\" class=\"sec sec-last\">\n<h3 id=\"__sec15title\">Ivermectin as a stem-cell cancer inhibitor<\/h3>\n<p id=\"__p28\" class=\"p p-first-last\">In 2009, Gupta\u00a0<em>et al.<\/em>\u00a0performed a high-throughput screening to discover selective inhibitors of cancer stem cells (CSCs), and found that salinomycin reduces the proportion of CSCs by &gt;100-fold relative to paclitaxel. Besides, they showed that salinomycin inhibits mammary tumor growth\u00a0<em>in vivo<\/em>, and that it induces increased epithelial differentiation of tumor cells accompanied by the loss of expression of breast CSC genes [<a id=\"__tag_670054642\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b66\" aria-expanded=\"false\" aria-haspopup=\"true\">66<\/a>]. As salinomycin is an antiparasitic drug for veterinary use only, our group searched similar compounds for human use that could also act as selective inhibitors of CSCs. Our results showed that ivermectin has high similarity with salinomycin (similarity of 0.78), and therefore we hypothesized that the antiparasitic drug ivermectin could also have similar biological properties as salinomycin [<a id=\"__tag_670054585\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b67\" aria-expanded=\"false\" aria-haspopup=\"true\">67<\/a>]. The results of this study showed that ivermectin has growth inhibitory effects upon MDA-MB-231 cells in the range of 0.2-8 \u00b5M, and as predicted, ivermectin preferentially inhibits the viability of CSCs-enriched populations (CD44<sup>+<\/sup>\/CD24<sup>&#8211;<\/sup>) and cells growing in spheroids, as compared to bulk cell population. The opposite pattern was observed with paclitaxel where the non-CSCs (CD44<sup>+<\/sup>\/CD24<sup>+<\/sup>) were sensitive to paclitaxel at nanomolar concentrations, while the inhibition of the stem cell subpopulation was only observed at higher drug concentrations. According with this, ivermectin reduces the expression of maintenance of the pluripotency and self-renewal markers Nanog, Oct4 and Sox2 at both mRNA and protein levels [<a id=\"__tag_670054599\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b67\" aria-expanded=\"false\" aria-haspopup=\"true\">67<\/a>]. A summary of the molecular mechanisms of the antitumor effects of ivermectin are shown in\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl02\/\" target=\"table\" rel=\"noopener\">Table 2<\/a>\u00a0and\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/figure\/fig02\/\" target=\"figure\" rel=\"noopener\">Figure 2<\/a>.<\/p>\n<\/div>\n<\/div>\n<div id=\"__sec16\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec16title\" class=\"head no_bottom_margin ui-helper-clearfix\">Antitumor effects of ivermectin, in vitro and in vivo<\/h2>\n<p id=\"__p29\" class=\"p p-first\">There are a number of\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0preclinical studies where ivermectin demonstrates its efficacy against a wide range of malignant conditions, including solid and hematological malignancies. In breast cancer, ivermectin has been studied in MDA-MB-435, MDA-MB-231, MDA-MB-468, MDA-MB-361, MCF-7, HS578T and SKBR3 cell lines where it demonstrates its ability to inhibit cell proliferation, induction of apoptosis, autophagy and reversion of tamoxifen resistance among other effects [<a id=\"__tag_670054620\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b51\" aria-expanded=\"false\" aria-haspopup=\"true\">51<\/a>,<a id=\"__tag_670054598\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b55\" aria-expanded=\"false\" aria-haspopup=\"true\">55<\/a>,<a id=\"__tag_670054658\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b60\" aria-expanded=\"false\" aria-haspopup=\"true\">60<\/a>,<a id=\"__tag_670054541\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b67\" aria-expanded=\"false\" aria-haspopup=\"true\">67<\/a>]. These effects are also reported in cancer cell lines from ovarian, prostate, head and neck, colon, and pancreas, as well as in melanoma [<a id=\"__tag_670054661\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b9\" aria-expanded=\"false\" aria-haspopup=\"true\">9<\/a>,<a id=\"__tag_670054573\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b45\" aria-expanded=\"false\" aria-haspopup=\"true\">45<\/a>,<a id=\"__tag_670054617\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b51\" aria-expanded=\"false\" aria-haspopup=\"true\">51<\/a>]. Similar results are also observed in a number of murine cancer cell lines including breast, melanoma and colon [<a id=\"__tag_670054623\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b51\" aria-expanded=\"false\" aria-haspopup=\"true\">51<\/a>,<a id=\"__tag_670054606\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b60\" aria-expanded=\"false\" aria-haspopup=\"true\">60<\/a>]. Two studies in glioblastoma cell lines show that ivermectin not only induces growth arrest and apoptosis, but induces anti-angiogenic effects [<a id=\"__tag_670054647\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b48\" aria-expanded=\"false\" aria-haspopup=\"true\">48<\/a>,<a id=\"__tag_670054591\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b58\" aria-expanded=\"false\" aria-haspopup=\"true\">58<\/a>], whereas two more studies extend these observation upon myeloblastic acute leukemia cell lines [<a id=\"__tag_670054616\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b41\" aria-expanded=\"false\" aria-haspopup=\"true\">41<\/a>,<a id=\"__tag_670054666\" class=\" bibr popnode tag_hotlink tag_tooltip\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b51\" aria-expanded=\"false\" aria-haspopup=\"true\">51<\/a>]. Of interest, the median concentration used for the\u00a0<em>in vitro<\/em>\u00a0treatment from all studies referred in\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl03\/\" target=\"table\" rel=\"noopener\">Table 3<\/a>\u00a0was 5 \u00b5M (0.01-100 \u00b5M) which is clinically achievable according with the pharmacokinetic data in humans shown in\u00a0<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl01\/\" target=\"table\" rel=\"noopener\">Table 1<\/a>. Regarding the\u00a0<em>in vivo<\/em>\u00a0evaluation of ivermectin (<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl04\/\" target=\"table\" rel=\"noopener\">Table 4<\/a>), this has been done in immune deficient mice using human acute myeloblastic leukemia, glioblastoma, breast and colon carcinoma, as well as in the murine lymphosarcoma cell line MDAY-D2. These studies show more than a 50% reduction in tumor volumes after ivermectin treatment, which varied from 10 to 42 days of treatment by either oral, intraperitoneal or intratumoral routes (more commonly intraperitoneal). The median dose employed was 5 mg\/kg (2.4-40 mg\/kg), which is equivalent to 0.40 mg\/kg in humans, a dose below to the highest dose safely used in human subjects evaluated so far (2 mg\/kg) (<a class=\"fig-table-link figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl01\/\" target=\"table\" rel=\"noopener\">Table 1<\/a>). Thus, the\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0results with ivermectin strongly suggest that its antitumor effects in cancer patients can be achieved at feasible doses.<\/p>\n<div id=\"tbl03\" class=\"table-wrap anchored whole_rhythm\">\n<h3>Table 3<\/h3>\n<div class=\"caption\">\n<p id=\"__p30\">Antitumor effects of ivermectin\u00a0<em>in vitro<\/em><\/p>\n<\/div>\n<div class=\"xtable\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140616582420704\">\n<table class=\"rendered small default_table\" frame=\"hsides\" rules=\"groups\" cellspacing=\"3\" cellpadding=\"2\">\n<thead>\n<tr>\n<th colspan=\"1\" rowspan=\"1\" align=\"left\">Tumor type<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Cell lines<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">[\u00b5M]<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"left\">Effects<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Reference<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"4\" align=\"left\" valign=\"top\">Human leukemia<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">OCI-AML2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5,<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"left\" valign=\"top\">Induces cell death through upregulation of ROS.<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b44\" aria-expanded=\"false\" aria-haspopup=\"true\">44<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">HL60<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">U937<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">15,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">K61a<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">20<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Prostate cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">DU145<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">PPC-1<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Human glioblastoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">U87<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1,<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Induces growth inhibition, apoptosis and anti-angiogenesis.<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b47\" aria-expanded=\"false\" aria-haspopup=\"true\">47<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">T98G<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"5\" align=\"left\" valign=\"top\">Ovarian cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">TYK-nu<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.1,<\/td>\n<td colspan=\"1\" rowspan=\"5\" align=\"left\" valign=\"top\">Inhibition of cell proliferation.<\/td>\n<td colspan=\"1\" rowspan=\"5\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b9\" aria-expanded=\"false\" aria-haspopup=\"true\">9<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">KOC7C<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">SKOV3<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">RMUG-S<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">100<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">HEI-193<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"6\" align=\"left\" valign=\"top\">Breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MDA-MB-435,<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5,<\/td>\n<td colspan=\"1\" rowspan=\"6\" align=\"left\" valign=\"top\">Stimulates autophagy and inhibition of cell proliferation.<\/td>\n<td colspan=\"1\" rowspan=\"6\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b54\" aria-expanded=\"false\" aria-haspopup=\"true\">54<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MDA-MB-231<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MDA-MB-468<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">15,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MDA-MB-361<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">20<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MCF-7<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">HS578T<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Murine breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">4T1.2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1,<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Induces apoptosis and necrosis. Induces autophagy.<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b50\" aria-expanded=\"false\" aria-haspopup=\"true\">50<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">DDHer2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">4,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">8,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Murine melanoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">C57BL\/6<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">16<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Inhibition of cell proliferation and clonogenic capacity.<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Murine colon adenocarcinoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">CT.26<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Increases the amount of ROS.<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Human breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MDA-MB-231<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MCF7<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">SKBR3<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Human melanoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">A2058<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">A375<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Human pancreatic cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">PANC1<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MiaPaca2<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Human prostate cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">DU145<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Human head and neck cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">A253<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Human leukemia<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MV411<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"4\" align=\"left\" valign=\"top\">Human colon cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">CC14<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.1,<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Inhibits cell proliferation and induces apoptosis.<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">CC36<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1,<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">Ls174T<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5,<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">HT29<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Human glioblastoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">DLD1<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">U251<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Human melanoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">SKMe12<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Murine breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">4T1<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.01,<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Inhibition of invasiveness and restoration of tamoxifen sensitivity.<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b59\" aria-expanded=\"false\" aria-haspopup=\"true\">59<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MMTV-Myc<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.1,<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Human breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">MDA-MB-231<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">1<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Inhibition of cell growth and metastases.<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">D3H2LN<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"6\" align=\"left\" valign=\"top\">Human breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"6\" align=\"center\" valign=\"top\">MDA-MB-231<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.2,<\/td>\n<td colspan=\"1\" rowspan=\"6\" align=\"left\" valign=\"top\">Preferentially inhibits cell viability and clonogenicity of the stem cell population.<\/td>\n<td colspan=\"1\" rowspan=\"6\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b67\" aria-expanded=\"false\" aria-haspopup=\"true\">67<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.4,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">0.8,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">1,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<div id=\"largeobj_idm140616582420704\" class=\"largeobj-link align_right\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/table\/tbl03\/?report=objectonly\" target=\"object\" rel=\"noopener\">Open in a separate window<\/a><\/div>\n<\/div>\n<div id=\"tbl04\" class=\"table-wrap anchored whole_rhythm\">\n<h3>Table 4<\/h3>\n<div class=\"caption\">\n<p id=\"__p31\">Antitumor effects of ivermectin in vivo<\/p>\n<\/div>\n<div class=\"xtable\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140616577991040\">\n<table class=\"rendered small default_table\" frame=\"hsides\" rules=\"groups\" cellspacing=\"3\" cellpadding=\"2\">\n<thead>\n<tr>\n<th colspan=\"1\" rowspan=\"1\" align=\"left\">Tumor type<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Tumor cell line<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Days of treat.<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Dose mg\/kg<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Mice<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"left\">Effects of Ivermectin<\/th>\n<th colspan=\"1\" rowspan=\"1\" align=\"center\">Ref.<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"4\" align=\"left\" valign=\"top\">Murine leukemia<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"center\" valign=\"top\">MDAY-D2<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"center\" valign=\"top\">10<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">3,<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"center\" valign=\"top\">NOD\/SCID mice<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"left\" valign=\"top\">Reduces tumor volume up to 70% in all models<\/td>\n<td colspan=\"1\" rowspan=\"4\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b44\" aria-expanded=\"false\" aria-haspopup=\"true\">44<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">5,<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">6<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">i.p.<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Human leukemia<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">K562<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\"><\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">3 oral<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\"><\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\"><\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">OCI-AML2<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Human glioblastoma<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">U87<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">21<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">40<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">SCID mice<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Reduces tumor volume up to 50%<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b47\" aria-expanded=\"false\" aria-haspopup=\"true\">47<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">T98G<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">i.p.<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Breast cancer<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">MDA-MB-231-GFP<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">10<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">2.4<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">NOD\/SCID mice<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"left\" valign=\"top\">Reduces tumor volume up to 60%<\/td>\n<td colspan=\"1\" rowspan=\"2\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b54\" aria-expanded=\"false\" aria-haspopup=\"true\">54<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">i.p.<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Human glioma<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">U87MG<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">42<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">3,<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">Balb\/c nude mice<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">Reduces tumor volume up to 50% at 3 mg\/kg.<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b64\" aria-expanded=\"false\" aria-haspopup=\"true\">64<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\">At 10 mg\/kg tumors were not detectable<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">i.t.<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"left\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Human colon cancer<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">LDL1<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">21<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">10<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">NMRI nude mice<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"left\" valign=\"top\">Reduces tumor volume up to 85% (LDL1 cell line). No effect is observed in the tumor TCF-independent cell line (CC14)<\/td>\n<td colspan=\"1\" rowspan=\"3\" align=\"center\" valign=\"top\">[<a class=\" bibr popnode\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#b57\" aria-expanded=\"false\" aria-haspopup=\"true\">57<\/a>]<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">CC14<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">i.p.<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\">HT29<\/td>\n<td colspan=\"1\" rowspan=\"1\" align=\"center\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<div class=\"tblwrap-foot\">\n<div id=\"idm140616584711360\">\n<p id=\"__p32\" class=\"p p-first-last\">i.t.: intratumoral. i.p.: intraperitoneal.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"__sec17\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec17title\" class=\"head no_bottom_margin ui-helper-clearfix\">Conclusions<\/h2>\n<p id=\"__p33\" class=\"p p-first-last\">The recognition that drug repositioning is a clever opportunity to accelerate the development of cancer drugs is increasing. So far, at least 235 clinically-approved, non-cancer drugs have proven antitumor activity either\u00a0<em>in vitro<\/em>,\u00a0<em>in vivo<\/em>, or even clinically. Among these, ivermectin, an antiparasitic compound of wide use in veterinary and human medicine, is clearly a strong candidate for repositioning, based on the fact that i) it is very safe, causing almost no side-effects other than those caused by the immune and inflammatory responses against the parasite in infected patients, and ii) it has proven antitumor activity in preclinical studies. On the other hand, it is now evident that the use of very selective \u201cunitargeted\u201d drugs is commonly associated to early development of resistance by cancer cells, hence the use of \u201cdirty\u201d or \u201cmultitargeted\u201d drugs is important to explore. In this sense, ivermectin has this potential as it modulates several targets such as the multidrug resistance protein (MDR), the Akt\/mTOR and WNT-TCF pathways, the purinergic receptors, the PAK-1 protein, certain cancer-related epigenetic deregulators such as SIN3A and SIN3B, RNA helicase activity, while stimulates chloride channel receptors leading to cell hyperpolarization, and down-regulates stemness genes to preferentially target cancer stem-cell like population, at least in breast cancer. Importantly, the\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0antitumor activities of ivermectin are achieved at concentrations that can be clinically reachable based on the human pharmacokinetic studies done in healthy and parasited patients. Thus, existing information on ivermectin could allow its rapid move into clinical trials for cancer patients.<\/p>\n<\/div>\n<div id=\"idm140616589819056\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"idm140616589819056title\" class=\"head no_bottom_margin ui-helper-clearfix\">Acknowledgements<\/h2>\n<div class=\"sec\">\n<p id=\"__p34\">Mandy Juarez is a student belonging to the Programa de Ciencias Bioqu\u00edmicas, UNAM. Alejandro Schcolnik-Cabrera is a student belonging to the Plan de Estudios Combinados en Medicina (PECEM), UNAM.<\/p>\n<\/div>\n<\/div>\n<div id=\"__sec18\" class=\"tsec bk-sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"__sec18title\" class=\"head no_bottom_margin ui-helper-clearfix\">Disclosure of conflict of interest<\/h2>\n<p id=\"__p35\" class=\"p p-first-last\">None.<\/p>\n<\/div>\n<div id=\"idm140616589816304\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" title=\"Go to other sections in this page\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5835698\/?fbclid=IwAR2YECuln5caIEFwDa4wGHwGuowValoKHhOP_g_SaHM7OftaaDAagYzXDr0#\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 id=\"idm140616589816304title\" class=\"head no_bottom_margin ui-helper-clearfix\">References<\/h2>\n<div id=\"reference-list\" class=\"ref-list-sec sec\">\n<div id=\"b1\" class=\"ref-cit-blk half_rhythm\">1.\u00a0<span class=\"element-citation\">Crump A. 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