{"id":80952,"date":"2021-08-27T17:17:06","date_gmt":"2021-08-27T21:17:06","guid":{"rendered":"http:\/\/stateofthenation.co\/?p=80952"},"modified":"2021-08-27T17:17:06","modified_gmt":"2021-08-27T21:17:06","slug":"is-this-why-graphene-oxide-was-put-into-the-covid-vaccines-for-connecting-to-5g-grid-magnetofection","status":"publish","type":"post","link":"http:\/\/stateofthenation.co\/?p=80952","title":{"rendered":"<h3><b><i>Is this why Graphene Oxide was put into the Covid vaccines for connecting to 5G Grid Magnetofection?<\/i><\/b><\/h3>"},"content":{"rendered":"<p><!--more--><a href=\"http:\/\/stateofthenation.co\/wp-content\/uploads\/2021\/08\/1000.webp\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/stateofthenation.co\/wp-content\/uploads\/2021\/08\/1000.webp\" alt=\"\" width=\"600\" height=\"360\" class=\"aligncenter size-full wp-image-80953\" srcset=\"http:\/\/stateofthenation.co\/wp-content\/uploads\/2021\/08\/1000.webp 600w, http:\/\/stateofthenation.co\/wp-content\/uploads\/2021\/08\/1000-300x180.webp 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p><a class=\"dcr-amp-1oc3zo5\" href=\"https:\/\/www.theguardian.com\/science\/neurophilosophy\">Neurophilosophy<\/a><\/p>\n<div>\n<h1 class=\"dcr-amp-1rcstkd\">Genetically engineered \u2018Magneto\u2019 protein remotely controls brain and behaviour<\/h1>\n<\/div>\n<div class=\"dcr-amp-1f5r9o4\" data-print-layout=\"hide\">\n<h3>\u201cBadass\u201d new method uses a magnetised protein to activate brain cells rapidly, reversibly, and non-invasively<\/h3>\n<p>Mo Costandi<br \/>\nThe Guardian<\/p>\n<\/div>\n<p>Researchers in the United States have developed a new method for controlling the brain circuits associated with complex animal behaviours, using genetic engineering to create a magnetised protein that activates specific groups of nerve cells from a distance.<\/p>\n<p>Understanding how the brain generates behaviour is one of the ultimate goals of neuroscience \u2013 and one of its most difficult questions. In recent years, researchers have developed a number of methods that enable them to remotely control specified groups of neurons and to probe the workings of neuronal circuits.<\/p>\n<div id=\"ad-2\" class=\"dcr-amp-brxoyj\" data-sort-time=\"1\"><iframe id=\"google_ads_iframe_2\" class=\"i-amphtml-fill-content\" title=\"3rd party ad content\" width=\"300\" height=\"250\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" sandbox=\"allow-forms allow-popups allow-popups-to-escape-sandbox allow-same-origin allow-scripts allow-top-navigation\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/div>\n<p>The most powerful of these is a method called <a href=\"https:\/\/www.google.co.uk\/search?q=site%3Ahttps%3A%2F%2Fwww.theguardian.com%2Fscience%2Fneurophilosophy%2F+optogenetics&amp;oq=site%3Ahttps%3A%2F%2Fwww.theguardian.com%2Fscience%2Fneurophilosophy%2F+optogenetics&amp;aqs=chrome..69i57j69i58.4360j0j4&amp;sourceid=chrome&amp;es_sm=91&amp;ie=UTF-8\">optogenetics<\/a>, which enables researchers to switch populations of related neurons on or off on a millisecond-by-millisecond timescale with pulses of laser light. Another recently developed method, called <a href=\"http:\/\/ijnp.oxfordjournals.org\/content\/ijnp\/18\/1\/pyu007.full.pdf\">chemogenetics<\/a>, uses engineered proteins that are activated by designer drugs and can be targeted to specific cell types.<\/p>\n<p>Although powerful, both of these methods have drawbacks. Optogenetics is invasive, requiring insertion of optical fibres that deliver the light pulses into the brain and, furthermore, the extent to which the light penetrates the dense brain tissue is severely limited. Chemogenetic approaches overcome both of these limitations, but typically induce biochemical reactions that take several seconds to activate nerve cells.<\/p>\n<aside class=\"dcr-amp-ossety\"><a class=\"dcr-amp-35mwn3\" href=\"https:\/\/www.theguardian.com\/science\/neurophilosophy\/2015\/mar\/24\/remote-control-brain-activity-nanoparticles\">Remote control of brain activity with heated nanoparticles<\/a><\/aside>\n<p>The new technique, developed in <a href=\"http:\/\/bio.as.virginia.edu\/people\/adg5h\">Ali G\u00fcler<\/a>\u2019s lab at the University of Virginia in Charlottesville, and <a href=\"http:\/\/www.nature.com\/neuro\/journal\/vaop\/ncurrent\/full\/nn.4265.html\">described<\/a> in an advance online publication in the journal <em>Nature Neuroscience<\/em>, is not only non-invasive, but can also activate neurons rapidly and reversibly.<\/p>\n<div id=\"ad-6\" class=\"dcr-amp-brxoyj\" data-sort-time=\"1\"><iframe id=\"google_ads_iframe_4\" class=\"i-amphtml-fill-content\" title=\"3rd party ad content\" src=\"https:\/\/2733592b81e266cfe45dbb2f8d3d343b.safeframe.googlesyndication.com\/safeframe\/1-0-38\/html\/container.html?n=0\" name=\"&quot;1-0-38;70496;&lt;!doctype\" data-mce-fragment=\"1\"><html><head><script>var jscVersion = 'r20210824';<\/script><script>var google_casm=[];<\/script><\/p>\n<style>a { color: #000000 }<\/style>\n<p><script>function su(id) {var a = document.getElementById(id);var b = (new Date()).getTime();if (a &amp;&amp; a.myt &amp;&amp; b) {var t = b - a.myt;if (window.css) {css(id,'clkt',t);return;}var bi = a.href.indexOf(&quot;&amp;clkt=&quot;);if (bi > 0) {var c = a.href.substring(0, bi+6); var d = a.href.substring(bi+6, a.href.length);var ei = d.indexOf(&quot;&amp;&quot;);var r = '';if (ei >= 0)r = d.substring(ei, d.length);a.href = c + t + r; } else {a.href += &quot;&amp;clkt=&quot; + t;}}}<\/script><script>window.dicnf = {};<\/script><script data-jc=&quot;40&quot; data-jc-version=&quot;r20210824&quot; data-jc-flags=&quot;[&amp;quot;x%278446&amp;#39;9efotm(&amp;amp;20067;&amp;gt;8&amp;amp;&amp;gt;`dopb\/%&amp;lt;1732261!=nehu`~&amp;quot;]&quot;>(function(){\/*  Copyright The Closure Library Authors. 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These methods represent an important advance \u2013 they have, for example, already been used to <a href=\"http:\/\/www.nature.com\/nm\/journal\/v21\/n1\/full\/nm.3730.html\">regulate blood glucose levels in mice<\/a> \u2013 but involve multiple components which have to be introduced separately.<\/p>\n<p>The new technique builds on this earlier work, and is based on a protein called TRPV4, which is <a href=\"https:\/\/www.theguardian.com\/science\/neurophilosophy\/2014\/mar\/03\/curry-cure\">sensitive to both temperature<\/a> and <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2930716\/\">stretching forces<\/a>. These stimuli open its central pore, allowing electrical current to flow through the cell membrane; this evokes nervous impulses that travel into the spinal cord and then up to the brain.<\/p>\n<p>G\u00fcler and his colleagues reasoned that magnetic torque (or rotating) forces might activate TRPV4 by tugging open its central pore, and so they used genetic engineering to fuse the protein to the paramagnetic region of ferritin, together with short DNA sequences that signal cells to transport proteins to the nerve cell membrane and insert them into it.<\/p>\n<figure class=\"dcr-amp-5bhc30\"><iframe class=\"i-amphtml-fill-content\" title=\"YouTube video\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/iHTpJNSNFlc?enablejsapi=1&amp;amp=1&amp;playsinline=1\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><figcaption class=\"dcr-amp-1e32u3u\"><\/figcaption><\/figure>\n<figure class=\"dcr-amp-5bhc30\"><figcaption class=\"dcr-amp-1e32u3u\"><span class=\"dcr-amp-19x4pdv\"><em>In vivo<\/em> manipulation of zebrafish behavior using Magneto. Zebrafish larvae exhibit coiling behaviour in response to localized magnetic fields. From Wheeler <em>et al<\/em> (2016). <\/span><\/figcaption><\/figure>\n<p>When they introduced this genetic construct into human embryonic kidney cells growing in Petri dishes, the cells synthesized the \u2018Magneto\u2019 protein and inserted it into their membrane. 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width=\"300\" height=\"250\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" sandbox=\"allow-top-navigation-by-user-activation allow-popups-to-escape-sandbox allow-forms allow-modals allow-pointer-lock allow-popups allow-same-origin allow-scripts\" allowfullscreen=\"allowfullscreen\" data-amp-3p-sentinel=\"0-32643805641318171233\"&gt;<\/iframe><\/div>\n<p>Next, the researchers inserted the Magneto DNA sequence into the genome of a virus, together with the gene encoding green fluorescent protein, and regulatory DNA sequences that cause the construct to be expressed only in specified types of neurons. They then injected the virus into the brains of mice, targeting the entorhinal cortex, and dissected the animals\u2019 brains to identify the cells that emitted green fluorescence. Using microelectrodes, they then showed that applying a magnetic field to the brain slices activated Magneto so that the cells produce nervous impulses.<\/p>\n<p>To determine whether Magneto can be used to manipulate neuronal activity in live animals, they injected Magneto into zebrafish larvae, targeting neurons in the trunk and tail that normally control an escape response. They then placed the zebrafish larvae into a specially-built magnetised aquarium, and found that exposure to a magnetic field induced coiling manouvres similar to those that occur during the escape response. (This experiment involved a total of nine zebrafish larvae, and subsequent analyses revealed that each larva contained about 5 neurons expressing Magneto.)<\/p>\n<aside class=\"dcr-amp-ossety\"><a class=\"dcr-amp-35mwn3\" href=\"https:\/\/www.theguardian.com\/science\/neurophilosophy\/2014\/dec\/22\/researchers-read-and-write-brain-activity-with-light\">Researchers read and write brain activity with light<\/a><\/aside>\n<p>In one final experiment, the researchers injected Magneto into the striatum of freely behaving mice, a deep brain structure containing dopamine-producing neurons that are involved in reward and motivation, and then placed the animals into an apparatus split into magnetised a non-magnetised sections. Mice expressing Magneto spent far more time in the magnetised areas than mice that did not, because activation of the protein caused the striatal neurons expressing it to release dopamine, so that the mice found being in those areas rewarding. This shows that Magneto can remotely control the firing of neurons deep within the brain, and also control complex behaviours.<\/p>\n<div id=\"ad-15\" class=\"dcr-amp-brxoyj\" data-sort-time=\"1\"><iframe id=\"google_ads_iframe_3\" class=\"i-amphtml-fill-content\" title=\"3rd party ad content\" width=\"300\" height=\"250\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" sandbox=\"allow-forms allow-popups allow-popups-to-escape-sandbox allow-same-origin allow-scripts allow-top-navigation\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/div>\n<p>Neuroscientist <a href=\"http:\/\/www.psmag.com\/health-and-behavior\/30-top-thinkers-30-neuroscience-hollywood-professor-wants-erase-memories-76417\">Steve Ramirez<\/a> of Harvard University, who uses optogenetics to <a href=\"http:\/\/www.smithsonianmag.com\/innovation\/meet-two-scientists-who-implanted-false-memory-mouse-180953045\/?no-ist\">manipulate memories<\/a> in the brains of mice, says the study is \u201c<a href=\"https:\/\/twitter.com\/okaysteve\/status\/707269086812708864\">badass<\/a>\u201d.<\/p>\n<p>\u201cPrevious attempts [using magnets to control neuronal activity] needed multiple components for the system to work \u2013 injecting magnetic particles, injecting a virus that expresses a heat-sensitive channel, [or] head-fixing the animal so that a coil could induce changes in magnetism,\u201d he explains. \u201cThe problem with having a multi-component system is that there\u2019s so much room for each individual piece to break down.\u201d<\/p>\n<p>\u201cThis system is a single, elegant virus that can be injected anywhere in the brain, which makes it technically easier and less likely for moving bells and whistles to break down,\u201d he adds, \u201cand their behavioral equipment was cleverly designed to contain magnets where appropriate so that the animals could be freely moving around.\u201d<\/p>\n<p>\u2018Magnetogenetics\u2019 is therefore an important addition to neuroscientists\u2019 tool box, which will undoubtedly be developed further, and provide researchers with new ways of studying brain development and function.<\/p>\n<h2><strong>Reference<\/strong><\/h2>\n<p>Wheeler, M. A., <em>et al<\/em>. (2016). Genetically targeted magnetic control of the nervous system. <em>Nat. Neurosci<\/em>., DOI: 10.1038\/nn.4265 [<a href=\"http:\/\/www.nature.com\/neuro\/journal\/vaop\/ncurrent\/full\/nn.4265.html\">Abstract<\/a>]<\/p>\n<p>___<br \/>\n<a href=\"https:\/\/amp.theguardian.com\/science\/neurophilosophy\/2016\/mar\/24\/magneto-remotely-controls-brain-and-behaviour\">https:\/\/amp.theguardian.com\/science\/neurophilosophy\/2016\/mar\/24\/magneto-remotely-controls-brain-and-behaviour<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-80952","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"http:\/\/stateofthenation.co\/index.php?rest_route=\/wp\/v2\/posts\/80952","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/stateofthenation.co\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/stateofthenation.co\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/stateofthenation.co\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/stateofthenation.co\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=80952"}],"version-history":[{"count":0,"href":"http:\/\/stateofthenation.co\/index.php?rest_route=\/wp\/v2\/posts\/80952\/revisions"}],"wp:attachment":[{"href":"http:\/\/stateofthenation.co\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=80952"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/stateofthenation.co\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=80952"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/stateofthenation.co\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=80952"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}